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Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

Identifieur interne : 000723 ( Main/Exploration ); précédent : 000722; suivant : 000724

Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

Auteurs : Ewa Gregoraszczuk [Pologne] ; Agnieszka Rak [Pologne] ; K. Kawalec [Pologne] ; Erik Ropstad [Norvège]

Source :

RBID : Pascal:08-0273628

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English descriptors

Abstract

The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.


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Le document en format XML

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<name sortKey="Gregoraszczuk, Ewa" sort="Gregoraszczuk, Ewa" uniqKey="Gregoraszczuk E" first="Ewa" last="Gregoraszczuk">Ewa Gregoraszczuk</name>
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<keywords scheme="KwdEn" xml:lang="en">
<term>Animals (MeSH)</term>
<term>Apoptosis</term>
<term>Apoptosis (drug effects)</term>
<term>Caspase 3 (metabolism)</term>
<term>Cell Survival (drug effects)</term>
<term>Cell death</term>
<term>Coculture Techniques (MeSH)</term>
<term>DDE</term>
<term>DDT</term>
<term>DDT (toxicity)</term>
<term>Dichlorodiphenyl Dichloroethylene (toxicity)</term>
<term>Estradiol (metabolism)</term>
<term>Exposure</term>
<term>Female (MeSH)</term>
<term>Flame Retardants (toxicity)</term>
<term>Flame retardant</term>
<term>Follicular cell</term>
<term>Granulosa Cells (drug effects)</term>
<term>Granulosa Cells (metabolism)</term>
<term>Halogenated Diphenyl Ethers (MeSH)</term>
<term>Indicators and Reagents (MeSH)</term>
<term>Insecticides (toxicity)</term>
<term>L-Lactate Dehydrogenase (metabolism)</term>
<term>Metabolite</term>
<term>Mixture</term>
<term>Ovarian Follicle (cytology)</term>
<term>Ovarian Follicle (drug effects)</term>
<term>Ovarian Follicle (metabolism)</term>
<term>Ovarian follicle</term>
<term>Ovary</term>
<term>Phenyl Ethers (toxicity)</term>
<term>Polybrominated Biphenyls (toxicity)</term>
<term>Secretion</term>
<term>Steroid</term>
<term>Steroids (metabolism)</term>
<term>Swine (MeSH)</term>
<term>Testosterone (metabolism)</term>
<term>Theca Cells (drug effects)</term>
<term>Theca Cells (metabolism)</term>
<term>Viability</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>1,1-Dichloro-2,2-bis(4-chlorophényl)éthylène (toxicité)</term>
<term>Animaux (MeSH)</term>
<term>Apoptose (effets des médicaments et des substances chimiques)</term>
<term>Caspase-3 (métabolisme)</term>
<term>Cellules de la granulosa (effets des médicaments et des substances chimiques)</term>
<term>Cellules de la granulosa (métabolisme)</term>
<term>Cellules thécales (effets des médicaments et des substances chimiques)</term>
<term>Cellules thécales (métabolisme)</term>
<term>DDT (toxicité)</term>
<term>Femelle (MeSH)</term>
<term>Follicule ovarique (cytologie)</term>
<term>Follicule ovarique (effets des médicaments et des substances chimiques)</term>
<term>Follicule ovarique (métabolisme)</term>
<term>Ignifuges (toxicité)</term>
<term>Indicateurs et réactifs (MeSH)</term>
<term>Insecticides (toxicité)</term>
<term>L-Lactate dehydrogenase (métabolisme)</term>
<term>Oestradiol (métabolisme)</term>
<term>Polybromobiphényles (toxicité)</term>
<term>Stéroïdes (métabolisme)</term>
<term>Suidae (MeSH)</term>
<term>Survie cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Techniques de coculture (MeSH)</term>
<term>Testostérone (métabolisme)</term>
<term>Éthers de polyhalogénophényle (MeSH)</term>
<term>Éthers phényliques (toxicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en">
<term>Caspase 3</term>
<term>Estradiol</term>
<term>L-Lactate Dehydrogenase</term>
<term>Steroids</term>
<term>Testosterone</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr">
<term>Follicule ovarique</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en">
<term>Ovarian Follicle</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en">
<term>Apoptosis</term>
<term>Cell Survival</term>
<term>Granulosa Cells</term>
<term>Ovarian Follicle</term>
<term>Theca Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr">
<term>Apoptose</term>
<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Follicule ovarique</term>
<term>Survie cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Granulosa Cells</term>
<term>Ovarian Follicle</term>
<term>Theca Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>Caspase-3</term>
<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Follicule ovarique</term>
<term>L-Lactate dehydrogenase</term>
<term>Oestradiol</term>
<term>Stéroïdes</term>
<term>Testostérone</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en">
<term>DDT</term>
<term>Dichlorodiphenyl Dichloroethylene</term>
<term>Flame Retardants</term>
<term>Insecticides</term>
<term>Phenyl Ethers</term>
<term>Polybrominated Biphenyls</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr">
<term>1,1-Dichloro-2,2-bis(4-chlorophényl)éthylène</term>
<term>DDT</term>
<term>Ignifuges</term>
<term>Insecticides</term>
<term>Polybromobiphényles</term>
<term>Éthers phényliques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Animals</term>
<term>Coculture Techniques</term>
<term>Female</term>
<term>Halogenated Diphenyl Ethers</term>
<term>Indicators and Reagents</term>
<term>Swine</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Animaux</term>
<term>DDT</term>
<term>Femelle</term>
<term>Indicateurs et réactifs</term>
<term>Stéroïde</term>
<term>Suidae</term>
<term>Sécrétion</term>
<term>DDE</term>
<term>Exposition</term>
<term>Ovaire</term>
<term>Cellule folliculaire</term>
<term>Mélange</term>
<term>Retardateur flamme</term>
<term>Métabolite</term>
<term>Follicule ovarien</term>
<term>Techniques de coculture</term>
<term>Viabilité</term>
<term>Apoptose</term>
<term>Mort cellulaire</term>
<term>Congénère</term>
<term>Polybromobiphényle dérivé</term>
<term>Phényle éther(polybromo)</term>
<term>Éthers de polyhalogénophényle</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.</div>
</front>
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<country>
<li>Norvège</li>
<li>Pologne</li>
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